The area of the slide shown here is near the feather edge, distal from the point of application. This is the best area on a wedge slide for observing cell morphology. The red blood cells should be touching but not overlapping; there should be no rouleau formation. The distribution of cells for a differential count is extremely important. The larger cells, such as segmented neutrophils and monocytes, tend to be concentrated on the sides and feather edge, while the smaller lymphocytes are seen in the center. Most technicians select a good area that is close to the feather edge, but where there is no distortion of the white cells and the red cells as previously described. They count across the slide and then move a little backwards toward the thicker end of the slide for their next row to count. Excellent descriptions of how to make a good slide are in Dacie and Lewis' Practical Haematology, and in Williams' Hematology (see Suggested Readings).
Course Section: 03. Blood Smears Stained with Wright's Stain
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