An easier stain to interpret than crystal violet is one described by Schwab and Lewis in 1969. They used a combined stain of 0.5% neutral red in 1.0% saline and an equal volume of blood for 60 seconds, followed by the addition of one volume of the mixture of 0.5% brilliant green in saline. A drop of the mixture is spread on a slide and observed under oil immersion without further manipulation. On this slide is a green Heinz body and also a red Howell-Jolly body in the cell in the center of the slide, and a green Heinz body in a cell towards the 12-o'clock position. There are two cells with red staining bodies below the center and at the 6-o'clock position. The red staining bodies can be Pappenheimer bodies, Howell-Jolly bodies, or reticulocytes. The green bodies are specifically Heinz bodies.
Course Section: 02. Inclusions Found in Red Blood Cells
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