In this slide is shown the bone marrow of the same patient as was presented in slide 16. This bone marrow has been stained for myeloperoxidase activity. The granulocytic cells have heavily stained clumps of granules, while the monocytic cells have fewer, scattered and discrete granules. The FAB investigators recommend two cytochemical tests for the diagnosis of all new cases of acute leukemia. These recommended tests are (1) myeloperoxidase activity or Sudan black staining, which are positive in nearly all cases of myelocytic leukemia, and (2) esterase activity for identifying monocytes. The latter assay recommended is naphthol AS-D acetate substrate and fast blue or fast blue BB salt for coupling the liberated product to form a colored compound. The assay is performed with and without NaF, which inhibits monocyte esterase activity.
Course Section: 09. Acute Myelomonocytic Leukemia - Monocytic Leukemia - and Erythroleukemia
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