This slide illustrates bone marrow taken for microscopic examination. Approximately 0.5 ml of blood which contains bone marrow particles is aspirated from the bone marrow cavity with a syringe. The bone marrow material is placed on the upper end of a microscope slide, which is supported and slanted at a 15-degree angle. The blood runs down to the end and the bone marrow particles stick to the glass. The particles, which are the consistency of soft cream cheese, are picked up with a white blood cell or Sahli pipette and placed onto a no. 1 thickness, square, 22-mm coverglass. Another coverglass is placed over the bone marrow and the two coverglasses are pulled apart. The preparation is air-dried, stained, and mounted on a glass slide. Regular glass slides may be used the same way in place of coverglasses. The main concern is to obtain bone marrow particles and make the slides before the marrow clots. Anticoagulants introduce artifactual change in the structure of marrow cells. The best preparations are made without an anticoagulant.
Course Section: 06. Microscopic Examination of the Bone Marrow
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